Assessment of a metabarcoding approach for the characterisation of vector-borne bacteria in canines from Bangkok, Thailand

Table 3 Bacterial NGS and cPCR agreement statistics

VBD	cPCR	Bacterial NGS		Total agreement (%)	Kappa^a (agreement statistic)	Kappa SE
VBD	cPCR	POS	NEG	Total agreement (%)	Kappa^a (agreement statistic)	Kappa SE
E. canis	POS	26	12	74	0.454 (Moderate)	0.091
E. canis	NEG	14	48	74	0.454 (Moderate)	0.091
A. platys	POS	12	0	87	0.581 (Moderate)	0.098
A. platys	NEG	13	75	87	0.581 (Moderate)	0.098
Mycoplasma spp.	POS	35	5	91	0.812 (Very good)	0.06
Mycoplasma spp.	NEG	4	56	91	0.812 (Very good)	0.06

^aKappa agreement level: K < 0.2, poor; 0.21–0.40, fair; 0.41–0.60, moderate; 0.61–0.80, good; 0.81–1.00, very good

ISSN: 1756-3305