Fig. 5

BspA and Pmp proteins localize predominantly to the ER and Golgi. The subcellular localizations of the positive control (TVAG_166850) and the candidate Pmp (TVAG_212570) and BspA (TVAG_240680) protein were analyzed using a specific antibody against the HA-tag. The protein localizations are shown (green) together with the nucleus (blue) which was stained using 4′,6-diamidin-2-phenylindol (DAPI). a The C-terminal tagged proteins which were used for adhesion assays constantly show a localization to the ER and Golgi apparatus. While shifting this tag position to the N-terminus has no influence on the positive control (which is still found to reside in the ER) it leads to a relocalization of both the Pmp and BspA to the lysosome. In contrast, removing the cytoplasmic tails (by replacing it with the HA-tag) did not lead to any localization shift compared to the C-terminally tagged proteins analyzed first. b We further checked if those localizations are influenced by the presence of host cells, but no change could be observed for T. vaginalis cells grown on VECs. White dashed lines highlight the periphery of the human cells. Scale-bars: a, 2 µm; b, 5µm