Fig. 3

CLSM images of subtegumental structures of Dibothriocephalus latus (heat-treated plerocercoids). Detail of lateral scolex part with thin β tubulin-IR fibres (straight arrows) reaching tegument with their terminal parts (a–c). Detail of lateral scolex part with subtegumental β tubulin-IR and 5-HT-IR bipolar neurons with short projections reaching tegument (sinuous arrows) and their long fibres running toward nerve plexus (d–f). Apical part of scolex with terminal parts of distinct fibres types. Note numerous β tubulin-IR fibres (straight arrow) and much less frequent type showing co-localisation of β tubulin-IR and 5-HT-IR (sinuous arrow) (g–i). Terminal parts of three types of fibre structures in most apical part of scolex. Note rare thin β tubulin-IR fibres (straight arrow), wider β tubulin-IR and 5-HT-IR type (sinuous arrows), which both partly resemble fibres on lateral side of the scolex, and finally, numerous wide, solely β tubulin-IR fibre-like structures (arrowheads) (j–o). Abbreviations: AF, anchoring fibril; AP, apical pore; FC, flame cell