Fig. 4

Effects of Sjfs800-specific siRNA transfection. a To determine the Sfs800-specific siRNA with the best interference efficiency in vitro as measured using qPCR, 28-dpi worms were transfected with one of three siRNAs (siRNA1-3) and were harvested 3 days later. The qPCR results showed the effects of siRNA1 were reduced by nearly 60% compared with those in the mock-transfected group (F_{(4, 10)} = 1525, P < 0.0001). b Effects of siRNA1 on Sjfs800 mRNA levels in worms were tested 10 days later. Sjfs800 mRNA levels were normalized to the endogenous control SjPSMD4. The qPCR analysis results showed that the Sjfs800 mRNA levels in the group transfected with Sjfs800 siRNA1 were reduced by approximately 60% compared with those in the scrambled siRNA-transfected group (F_{(2, 6)} = 1251, P < 0.0001). Asterisks show statistical differences (**P < 0.01) tested by one-way ANOVA, compared to the mock group. Data are expressed as the mean ± SEM of three independent experiments (three biological replicates)