Table 1 Quantitative PCR primers (5’ to 3’)
Gene name | Gene ID | Sense primer | Anti-sense primer |
|---|---|---|---|
Unknown | 226471383 | ATTCACCACAACCCACT | TATTGCCACCTCCACTT |
Trematode eggshell synthesis | 226473101 | ATCCACGTCTTACCAGA | AGCAGCGATACTACCTT |
Superoxide dismutase | 226474963 | CCATTCAACATGCGTCC | CTGCCTTCATCTGGATTTCT |
Keratin 9 | 226477615 | ACAGCTACGGAAATGC | CAATAATAGGAGGGTGC |
Prostatic spermine-binding protein | 226477985 | GATTATGCTGATGTGAG | CGTTTGATTCGTGTCTA |
Myoglobin 1 | 226487201 | TCAGGGACTTGATGCTA | CAACTGGTCGAGTTCTAT |
Annexin | 226469397 | TCCATTCGGTTTACTCT | TTCAGCAATGTCCCTAG |
Cytochrome b | 56046805 | AAGAAGTTGGTGGTGG | GTGCCTTTGAGGTTGTCC |
Female-specific protein 800 | 60600619 | TGGAAACGAAAGTGAG | CTGGAATTGAAAGGACC |
Tetraspanin-1 | 56757404 | AAGGTAAAGGTGGTAGC | CAATGAATGCCGATAAG |
Tyrosinase precursor | 56757710 | TGGTGTTTGTTTCCCTA | CTCTATTACCACCTCTTTGA |
Putative transmembrane protein | 56757833 | TAGTTGGGAGACTTTGC | TACATTTCGGATTGCTG |
Multivesicular body protein 5 | 226467851 | TTGCTTCAAGGCGGCT | CCGTGTTGTTTATTGGGAC |
Unknown gene | 226477693 | TGGTGGTCCAGATTGTT | CATAGTAGTCATTTCCGTAG |
Egghell precursor protein | 226478597 | ATGACTACAACTCCGACTAC | CTCTGACATCTAAACGACCA |