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Table 1 Rhipicephalus (B.) microplus transmission of the B. bovis S17.2 and S18.2 vaccine strains, and the S17.2 clonal line parasite population

From: Investigation into limiting dilution and tick transmissibility phenotypes associated with attenuation of the S24 vaccine strain

Group

B. bovis strains

B. bovis Bv80 genotype classification according to alleles and base pairs

No. of cattle used for tick feeding

Parasitaemia during dropping of engorged females (%)

Percentage female ticks hemolymph positive for kinetes out of 20 examined

No. of cattle each infested with ± 20,000 larvae

B. bovis genotype(s) detected following transmission

1

S17.2 (S16)a

A558; A627; A645; B585

2

0.20–1.5

95

1

A558; A627; A645; B585

    

0.08–1.0

100

1

A558; A627; A645; B585

2

S18.2 (S17.2)a

A645; B585

2

0.60–1.7

100

1

A645; B585

    

0.20–1.2

50

1

A645; B585

3

Not infected

–

1

–

0

1

No transmission

4

9626-S17.2-cl (originating from 3 parasites of S17.2)a

A558

2

0.10–1.0; 0.09–0.8

0; 0

1; 1

No transmission

  1. aStabilate used for infection