Identification of Trypanosoma brucei gambiense in naturally infected dogs in Nigeria

Parasites & Vectors

Table 2 PCR for detection of African trypanosomes

Target taxon/gene	Primer name	Primer sequence (5’–3’)	Annealing temperature (°C)	Amplicon size (bp)	Reference
Trypanosoma ITS1	TRYP3	TGCAATTATTGGTCGCGC	54	Various sizes according to species	[26]
Trypanosoma ITS1	TRYP4	CTTTGCTGCGTTCTT	54	Various sizes according to species	[26]
Subgenus Trypanozoon 177-bp satellite repeat	TBR1	GAATATTAAACAATGCGCAG	60	164 (monomer)	[27, 32]
Subgenus Trypanozoon 177-bp satellite repeat	TBR2	CCATTTATTAGCTTTGTTGC	60	164 (monomer)	[27, 32]
T. congolense savannah 350-bp satellite repeat	TCS1	CGAGAACGGGCACTTTGCGA	60	316 (monomer)	[27]
T. congolense savannah 350-bp satellite repeat	TCS2	GGACAAACAAATCCCGCACA	60	316 (monomer)	[27]
T. evansi Type A kDNA minicircle	EVA1	ACATATCAACAACGACAAAG	60	139	[33]
T. evansi Type A kDNA minicircle	EVA2	CCCTAGTATCTCCAATGAAT	60	139	[33]
T. b. gambiense Group 1 TgsGP gene	TgsGP-F	GCTGCTGTGTTCGGAGAGC	50	308	[28]
T. b. gambiense Group 1 TgsGP gene	TgsGP-R	GCCATCGTGCTTGCCGCTC	50	308	[28]
T. b. gambiense Group 1 AnTat 11.17 VSG gene	AnTA-outer	CACAGACGACAGAAGCGATA	50	653	[29]
	AnTB-outer	GAAAGTGGGAGTTGTTGCTC	50	653
	AnTC-inner	GCCTTCGAAGACACAAGCAG	50	381
	AnTD-inner	XCGTCGTGCTGAAGTCTCCTG	50	381

ISSN: 1756-3305