Table 1 Sensitivity, specificity, accuracya, positive predictive value and negative predictive value of qPCR on pooled samples as compared to individual sample-based qPCR, for each one of the soil-transmitted helminth-specific qPCR assays, for both laboratories: Natural History Museum (NHM) and Smith College (SC)
Species | No. of pools tested | Sensitivity (95% CI) | Specificity (95% CI) | Accuracy (95% CI)Â | PPV (95% CI) | NPV (95% CI) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NHM | SC | NHM | SC | P b | NHM | SC | P b | NHM | SC | P b | NHM | SC | NHM | SC | |
N. americanus c | 66 | – | 0.71 (0.52–0.86) | – | < 0.001 | 1.00 (0.90–1.00) | – | 1 | 0.86 (0.84–0.89) | – | 0.01 | 1.00 (0.85–1.00) | – | 0.80 (0.65–0.90) | – |
A. lumbricoides | 72 | 56 | 0.69 (0.48–0.86) | 1.00 (0.91–1.00) | < 0.001 | 1.00 (0.92–1.00) | 1.00 (0.79–1.00) | 1 | 0.89 (0.87–0.91) | 1.00 (1.00–1.00) | 0.03 | 1.00 (0.81–1.00) | 1.00 (0.91–1.00) | 0.85 (0.73–0.93) | 1.00 (0.79–1.00) |
T. trichiura | 88 | 56 | 0.65 (0.50–0.79) | 0.80 (0.64–0.91) | 0.13 | 0.98 (0.87–1.00) | 1.00 (0.79–1.00) | 0.76 | 0.81 (0.77–0.84) | 0.80 (0.75–0.85) | 0.93 | 0.97 (0.83–1.00) | 1.00 (0.89–1.00) | 0.72 (0.58–0.83) | 0.67 (0.45–0.84) |
A. ceylanicum | 60 | 36 | 0.82 (0.60–0.95) | 1.00 (0.79–1.00) | 0.07 | 0.95 (0.82–0.99) | 1.00 (0.83–1.00) | 0.64 | 0.90 (0.88–0.92) | 1.00 (1.00–1.00) | 0.14 | 0.90 (0.68–0.99) | 1.00 (0.79–1.00) | 0.90 (0.76–0.97) | 1.00 (0.83–1.00) |
S. stercoralis | 60 | 36 | 0.70 (0.35–0.93) | 1.00 (0.40–1.00) | 0.22 | 0.96 (0.86–1.00) | 0.81 (0.64–0.93) | 0.03 | 0.92 (0.90–0.94) | 0.70 (0.61–0.79) | 0.01 | 0.78 (0.40–0.97) | 0.40 (0.12–0.74) | 0.94 (0.84–0.99) | 1.00 (0.87–1.00) |