Pooling as a strategy for the timely diagnosis of soil-transmitted helminths in stool: value and reproducibility

Parasites & Vectors

Table 2 Pearson’s correlation values between individual and pooled qPCR-results (P-value) at both Smith College (SC) and Natural History Museum (NHM)

Species	No. of positive samples correctly identified through pooling Field samples		Pearson’s r-value (P-value) Field samples
Species	NHM	SC	NHM	SC
N. americanus ^a	22		0.86 (P < 0.001)	–
A. lumbricoides	18	40	0.75 (P < 0.001)	0.86 (P < 0.001)
T. trichiura	30	32	0.41 (P = 0.025)	0.32 (P = 0.070)
An. ceylanicum	18	16	0.93 (P < 0.001)	0.92 (P < 0.001)
S. stercoralis	7	4	0.97 (P < 0.001)	− 0.07 (P = 0.926)

^a‘Seeding’ experiment results (available only at SC): 40 pools tested and identified as positive, Pearson’s r-value -0.26 (P = 0.112)

ISSN: 1756-3305