Fig. 2

Expression profiles of SHMT mRNA and protein in A. aegypti. a–f mRNA levels of SHMT in diverse tissues at the following time points: a 72 h post-eclosion (PE); b 6 h post-blood-meal (PBM); c 12 h PBM; d 24 h PBM; e 36 h PBM; f 72 h PBM. g mRNA levels of SHMT in the whole body of adult females at diverse time points. h Western blotting analysis of temporal expression of SHMT in the whole body of adult females. i SHMT protein levels in h were quantified following normalization to TUBULIN. Nuclear RNA RPS7 was used as a loading control in all qRT-PCR assays (a–g). The qRT-PCR data are presented as mean ± SEM. TUBLIN was used as the loading control in western blot. Abbreviations: RE, relative expression; HD, head; MG, midgut; FB, fat body; OV, ovary; LO, leftover of the whole body