Fig. 4

Phylogenetic analysis of gGAPDH sequences from trypanosome species detected in Nigerian tsetse. a gGAPDH nucleotide sequences of 27 field samples and 10 reference sequences were aligned as described under methods and a 677 bp stretch extracted. A Neighbour-Joining tree was calculated using complete gap deletion and tested with 700 bootstrap replications using MEGA6 [24]. b Phylogenetic relationships of the respective translated protein sequences were inferred by the Neighbour-Joining tree as described before. Note the diversity of T. grayi sequences on nucleotide and amino acid level. Reference sequences are marked by black circles and accession numbers are indicated after each sequence if available. Numbers of the field sequences reference the fly identification number. Gut and proboscis state the origin of the DNA amplicon. Abbreviations: EA, East Africa; WA, West Africa; SA, South America; Gpp, G. palpalis palpalis; Gms, G. morsitans submorsitans; Gt, G. tachinoides; Gsp, Glossina spp