Table 1 Primer sequences used in this study and their sources
From: Genetic diversity of trypanosome species in tsetse flies (Glossina spp.) in Nigeria
Primer name | Nucleotide sequence (5′–3′) | Amplicon size (bp) | Source |
|---|---|---|---|
ITS1-out2 | CTTTGCTGCGTTCTT | Variable (100–650) | [21] |
ITS1-out1 | TGCAATTATTGGTCGCGC | ||
ITS1-in1 | TAGAGGAAGCAAAAG | ||
ITS1-in2 | AAGCCAAGTCATCCATCG | ||
gGAPDH-out | TTYGCCGYATYGGYCGCATGG | 816 | [22] |
gGAPDH-out | ACMAGRTCCACCACRCGGTG | ||
gGAPDH-in | GCSTAYCAGATGAAGTACGAC | This study | |
gGAPDH-in | GTTYTGCAGSGTCGCCTTGG | [22] | |
TGR-out1 | TGGCAGACACATACCTGCCA | 526 | [20] |
TGR-out2 | TGGGGATTACGGATGAAAC | ||
TGR-in1 | TTAAGGAGGCGCTCAGGTTC | ||
TGR-in2 | TGTGCATATACGTCTATG | ||
TCON-out1 | TGCAATTATTGGTCGCGC | Variable (681–781) | [20] |
TCON-out2 | TGTTGGTCGACACTGAGA | ||
TCON-in1 | TCGCGTGTCTCACGT | ||
TCON-in1 | TCAAAGATTGGGCAATGT |