Vector competence is strongly affected by a small deletion or point mutations in bluetongue virus

Parasites & Vectors

Table 1 Overview of mutations in BTV11(S1²⁶) variants. BTV11(S1²⁶) was passed on BSR or KC cells as described. Rescued virus on KC cells (p3r) and variants after three virus passages (p3) on BSR or KC cells were completely sequenced, whereas virus variants after three additional virus passages (p6 and p3r+p6) were partially sequenced to confirm previously observed mutations. Mutations associated with adaptation to KC cells are in bold

^a ±50% or mixed nucleotides for each genome segment S1–10
^b < 50% or minor nucleotide mutations for each genome segment S1–10
Notes: Nucleotide positions are according to positions in the respective genome segments. aa mutations are shown in the right column of each passaged BTV11 variant. Note: mutation A981G in S2[VP2]¹¹ resulting in aa mutation E321G was also found for BTV11kc
Abbreviations: nd, genome segments of p6 and p3r+p6 without mutations in previous variants were not sequenced; –, no mutations

ISSN: 1756-3305