Fig. 1

Isolation and physical characterization of EVs from Acanthamoeba castellanii. a The flow chart of EV isolation from the excretory-secretory products of A. castellanii. b Morphological characterization of EVs by TEM. Arrows indicate isolated EVs stained with uranyl acetate. The enlarged region shows the EVs with a double membrane structure. c Representative nanoparticle tracking analysis (NTA) plot showing the distribution of size and concentration of EVs