Fig. 3
Cytopathogenicity of EVs from Acanthamoeba castellanii on C6 cells. a Rat glial C6 cells were cocultured with EVs from A. castellanii, and the CPE was evaluated by Giemsa staining after cocultivation for 24Â h. The data are representative of three independent experiments. b Light micrographs show the CPE of C6 cells produced by EVs from A. castellanii after cocultivation for 4Â h, 8Â h, 12Â h, 16Â h and 20Â h