Parasites & Vectors

Table 1 Forward and reverse primer sequences used for the amplification of Hematodinium by PCR. Each PCR run included initial denaturation and final extension steps, according to the first and final temperatures, respectively, noted in the thermocycler settings

From: Spatial and temporal disease dynamics of the parasite Hematodinium sp. in shore crabs, Carcinus maenas

Primers				Thermocycler settings			Amplicon size (bp)	References
Direction	Name	Sequence (5′–3′)	Final concentration (µM)	Temperature (°C)	Time	No. of cycles	Amplicon size (bp)	References
Forward	Hemat-F-1487	CCTGGCTCGATAGAGTTG	0.5	94	10 min	30	187	[57]
Reverse	Hemat-R- 1654	GGCTGCCGTCCGAATTATTCAC		94	15 s
				54	15 s
				72	30 s
				72	10 min
Forward	18SF2	CAGTTTCTGGAAGTGGCAGCTG	1	94	1 min	35	480	[58, 59]
Reverse	Hem3R	TAACCCGAGCCGAGGCATTCA		94	1 min
				58	1 min
				72	1 min
				72	10 min
Forward	18SF2	CAGTTTCTGGAAGTGGCAGCTG	0.5	94	1 min	35	380	[58]
Reverse	ITS R1	GAAGGGAAGGGGAGAAGAAGC		94	30 s
				57	1 min
				72	1 min
				72	7 min

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ISSN: 1756-3305

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