Primers | Thermocycler settings | Amplicon size (bp) | References | |||||
---|---|---|---|---|---|---|---|---|
Direction | Name | Sequence (5′–3′) | Final concentration (µM) | Temperature (°C) | Time | No. of cycles | ||
Forward | Hemat-F-1487 | CCTGGCTCGATAGAGTTG | 0.5 | 94 | 10 min | 30 | 187 | [57] |
Reverse | Hemat-R- 1654 | GGCTGCCGTCCGAATTATTCAC | 94 | 15 s | ||||
54 | 15 s | |||||||
72 | 30 s | |||||||
72 | 10 min | |||||||
Forward | 18SF2 | CAGTTTCTGGAAGTGGCAGCTG | 1 | 94 | 1 min | 35 | 480 | |
Reverse | Hem3R | TAACCCGAGCCGAGGCATTCA | 94 | 1 min | ||||
58 | 1 min | |||||||
72 | 1 min | |||||||
72 | 10 min | |||||||
Forward | 18SF2 | CAGTTTCTGGAAGTGGCAGCTG | 0.5 | 94 | 1 min | 35 | 380 | [58] |
Reverse | ITS R1 | GAAGGGAAGGGGAGAAGAAGC | 94 | 30 s | ||||
57 | 1 min | |||||||
72 | 1 min | |||||||
72 | 7 min |