Fig. 4From: Soluble egg antigen of Schistosoma japonicum induces pyroptosis in hepatic stellate cells by modulating ROS productionROS generation was elevated in S. japonicum-infected liver tissues and SEA-stimulated HSCs. a Representative fluorescence images and statistical analysis results of ROS generation probed by dihydroethidium (DHE) in liver tissue from normal mice and mice at 6 weeks post-infection. Scale-bars: 100 μm. b Primary cultured HSCs were not stimulated or were stimulated with 50 μg/ml SEA for 24 h, and representative fluorescence images of ROS generation probed by DCFH-DA were obtained under a confocal microscope. Scale-bars: 20 μm. c The mean fluorescence intensity (MFI) of intracellular ROS was examined and analysed by flow cytometry in HSCs not treated or treated with 50 μg/ml SEA for 24 h (n = 4). ***P < 0.001Back to article page