Fig. 5
A ROS scavenger mitigated SEA-induced caspase-1 activation and pyroptosis in HSCs. a The relative mRNA levels of caspase-1 were determined by qPCR in HSCs after challenge with 50, 100 or 200 μM H2O2. b The effects of the ROS scavenger NAC on the mRNA levels of caspase-1 in HSCs stimulated by SEA were determined by qPCR. c The effects of NAC on the protein levels of caspase-1 in HSCs were determined by Western blot analysis and quantitative analysis (n = 4). d Pyroptotic HSCs labelled with caspase-1 probe and PI were detected by flow cytometry. **P < 0.01, ***P < 0.001