Criteria | Techniques employed to demonstrate the criteria |
---|---|
Sufficient populations of immuno-naïve (susceptible) NHPs | Antibody detection |
Sufficient viraemia in the NHP to infect feeding mosquitoes | Viral isolation from blood |
Viral genome detection from blood | |
Antibody detection | |
Sufficient populations of spatially and temporally coincident competent (capable of viral transmission) infected mosquitoes that feed on NHPs | Mosquito capture and identification |
Virus detection in mosquitoes | |
Blood-meal analysis of fed mosquitoes | |
Observations of NHPs being fed upon by forest mosquitoes | |
Viral transmission experiments (between NHPs and mosquitoes) |