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Table 1 Criteria and techniques used to establish the presence of arboviral sylvatic cycles

From: Sylvatic cycles of arboviruses in non-human primates

Criteria Techniques employed to demonstrate the criteria
Sufficient populations of immuno-naïve (susceptible) NHPs Antibody detection
Sufficient viraemia in the NHP to infect feeding mosquitoes Viral isolation from blood
Viral genome detection from blood
Antibody detection
Sufficient populations of spatially and temporally coincident competent (capable of viral transmission) infected mosquitoes that feed on NHPs Mosquito capture and identification
Virus detection in mosquitoes
Blood-meal analysis of fed mosquitoes
Observations of NHPs being fed upon by forest mosquitoes
Viral transmission experiments (between NHPs and mosquitoes)