Table 1 Primer sequences for real-time PCR detection of kdr mutations V1016I and F1534C
Primer | Primer sequence (5’-3’) |
|---|---|
Val1016f | GCGGGCGGCGGGGGCGGGGCCACAAATTGTTTCCCACCCGCACCGG |
Ile1016f | GCGGGCACAAATTGTTTCCCACCCGCACTGA |
Ile1016r | TGATGAACCSGAATTGGACAAAAGC |
Cys1534+f | GCGGGCAGGGCGGCGGGGGCGGGGCCTCTACTTTGTGTTCTTCATCATGTG |
Phe1534+f | GCGGGCTCTACTTTGTGTTCTTCATCATATT |
Phe1534+r | TCTGCTCGTTGAAGTTGTCGAT |