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Table 1 PCR reaction using single worm lysate

From: From the feces to the genome: a guideline for the isolation and preservation of Strongyloides stercoralis in the field for genetic and genomic analysis of individual worms

Component

Amount

Single worm lysate

2.0 µl

Forward primer (10 μM)

0.5 µl

Reverse primer (10 μM)

0.5 µl

dNTPs (10 mM each)

0.4 µl

Taq DNA polymerase

0.3 µl (1.5 units)

10× ThermoPol Reaction Buffer

2.5 µl

H2O

18.8 µl

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Parasites & Vectors

ISSN: 1756-3305

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