Region amplified
|
Primer
|
Sequence (5′–3′)
|
Annealing T (°C)
|
Product size (bp)
|
Reference
|
---|
18S HVR-I
|
Forward
|
ZS6492; SSU18Aa
|
AAACATGAAACCGCGGAAAG; AAAGATTAAGCCATGCATG
|
52
|
825 (ZS6492 and SSU26R); 862 (SSU18A and SSU26R)
|
[41]
|
Reverse
|
SSU26Ra
|
CATTCTTGGCAAATGCTTTCG
|
Sequencing
|
SSU9Ra
|
AGCTGGAATTACCGCGGCTG
| |
[40]
|
18S HVR-IV
|
Forward
|
18SP4F
|
GCGAAAGCATTTGCCAA
|
57
|
712
|
[29]
|
Reverse
|
18SPCR
|
ACGGCCGGTGTGTAC
|
Sequencing
|
ZS6269
|
GTGGTGCATGGCCGTTC
|
[35]
|
ytP 274
|
Forward
|
TJ6026
|
CAGGACCACCTGGACAAGTT
|
54
|
543
|
[26]
|
Reverse
|
TJ6027
|
CTTTCCATCCTGATGCCACT
|
Sequencing
|
TJ6026
|
CAGGACCACCTGGACAAGTT
|
- aIn some publications, SSU18A is referred to as SSUA (e.g.[40, 43]); in publications from our department, SSU18A, SSU26R and SSU9R are also referred to as RH5401, RH5402 and RH5403, respectively [35, 44]
- Abbreviation: T, temperature