Region amplified | Primer | Sequence (5′–3′) | Annealing T (°C) | Product size (bp) | Reference | |
---|---|---|---|---|---|---|
18S HVR-I | Forward | ZS6492; SSU18Aa | AAACATGAAACCGCGGAAAG; AAAGATTAAGCCATGCATG | 52 | 825 (ZS6492 and SSU26R); 862 (SSU18A and SSU26R) | [41] |
Reverse | SSU26Ra | CATTCTTGGCAAATGCTTTCG | ||||
Sequencing | SSU9Ra | AGCTGGAATTACCGCGGCTG | Â | [40] | ||
18S HVR-IV | Forward | 18SP4F | GCGAAAGCATTTGCCAA | 57 | 712 | [29] |
Reverse | 18SPCR | ACGGCCGGTGTGTAC | ||||
Sequencing | ZS6269 | GTGGTGCATGGCCGTTC | [35] | |||
ytP 274 | Forward | TJ6026 | CAGGACCACCTGGACAAGTT | 54 | 543 | [26] |
Reverse | TJ6027 | CTTTCCATCCTGATGCCACT | ||||
Sequencing | TJ6026 | CAGGACCACCTGGACAAGTT |