From the feces to the genome: a guideline for the isolation and preservation of Strongyloides stercoralis in the field for genetic and genomic analysis of individual worms

Table 2 Primers, annealing temperatures and product size

Region amplified	Primer		Sequence (5′–3′)	Annealing T (°C)	Product size (bp)	Reference
18S HVR-I	Forward	ZS6492; SSU18A^a	AAACATGAAACCGCGGAAAG; AAAGATTAAGCCATGCATG	52	825 (ZS6492 and SSU26R); 862 (SSU18A and SSU26R)	[41]
	Reverse	SSU26R^a	CATTCTTGGCAAATGCTTTCG		825 (ZS6492 and SSU26R); 862 (SSU18A and SSU26R)	[41]
	Sequencing	SSU9R^a	AGCTGGAATTACCGCGGCTG			[40]
18S HVR-IV	Forward	18SP4F	GCGAAAGCATTTGCCAA	57	712	[29]
	Reverse	18SPCR	ACGGCCGGTGTGTAC			[29]
	Sequencing	ZS6269	GTGGTGCATGGCCGTTC			[35]
ytP 274	Forward	TJ6026	CAGGACCACCTGGACAAGTT	54	543	[26]
	Reverse	TJ6027	CTTTCCATCCTGATGCCACT
	Sequencing	TJ6026	CAGGACCACCTGGACAAGTT

^aIn some publications, SSU18A is referred to as SSUA (e.g.[40, 43]); in publications from our department, SSU18A, SSU26R and SSU9R are also referred to as RH5401, RH5402 and RH5403, respectively [35, 44]
Abbreviation: T, temperature