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Table 6 Summary of proposed minimum data recording and reporting standards for studies investigating O. tsutsugamushi in vectors and non-human hosts

From: Scrub typhus ecology: a systematic review of Orientia in vectors and hosts

Topic Checklist item Notes
Dates Vector and host collection dates Month(s) and year(s). Where collections are made monthly, data should be reported monthly to inform seasonality of disease
Location Study site GPS coordinates and administrative divisions 1 to 4a Spelling, transliteration and changes over time can be avoided by using GPS coordinates
Sample collection and storage method Hosts trapping technique. Vectors collection method. Sample storage medium and conditions For collection of free-living vectors, provide details on methodsc
Host species All hosts should be identified to genus and species Reference up to date nomenclature
Vector species All vectors should be identified to genus and species where possibleb Record identification method and reference up to date nomenclature
Laboratory methods Provide details of laboratory methodology Where several methods are used, ensure data clearly separated by test
Sample type List Where several sample types tested, separate results. Where tissues are pooled, provide details
Testing hosts Numbers tested and numbers positive Avoid pooling samples from different hosts (and species). Avoid pooling samples from different sites
Testing vectors Numbers tested and numbers positive. If pooled give approximate number or range per pool Avoid pooling samples from different sites
Host vector infestation rate Proportion of hosts infested by the vector. Divide by host species and location If possible, provide vector species composition
Host vector index (chigger index: average number of chiggers/host) Vector (chigger) index. Divide by host species and location If possible, provide vector species composition
Ecology Habitat description; mean, minimum and maximum temperature; rainfall; humidity; soil type Note differences between study sites where applicable
Study ethics Provide details of appropriate animal handling and euthanasia (if used) protocol and approval Local, national and international standards may apply
  1. aAdministrative division categorization varies widely from country to country. The equivalent of province, district, sub-district and town or village should be given
  2. bAs a minimum, genus must be recorded. For pooled vectors, the majority of constituent genus/species should be recorded
  3. cBlack plate, Berlese or Tullgren funnels or other techniques