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Table 2 Effect of the vaccination with recombinant proteins CHI-GST, TSP1-GST + TSP2, RPP0, and PK4 administered in Montanide ISA 50 V2 on O. erraticus specimens fed on control and vaccinated rabbits

From: In silico selection of functionally important proteins from the mialome of Ornithodoros erraticus ticks and assessment of their protective efficacy as vaccine targets

Parameter Developmental stage Control Merged Control Montanide Control SjGST CHI-GST (% reduction) TSP1-GST + TSP2 (% reduction) RPP0 (% reduction) PK4 (% reduction)
Ingested blood (mg) Males 3.7 ± 0.5 3.7 ± 0.1 3.7 ± 0.7 3.6 ± 0.1 (2.7) 3.8 ± 0.2 4.1 ± 0.2 3.4 ± 0.3 (8.1)
Females 13.0 ± 1.1 13.0 ± 3.1 13.0 ± 1.2 13.3 ± 0.6 13.3 ± 1.2 12.8 ± 2.0 (1.5) 12.5 ± 0.2 (3.8)
Nymphs-3 3.3 ± 0.5 3.2 ± 0.4 3.5 ± 0.5 3.0 ± 0.5 (9.1) 3.9 ± 0.4 3.3 ± 0.5 3.1 ± 0.7 (6.1)
Mortality (%) Males 0.6 ± 1.4 0 ± 0 1.1 ± 1.8 0 ± 0 0 ± 0 0 ± 0 0 ± 0
Females 0 ± 0 0 ± 0 0 ± 0 0 ± 0 0 ± 0 2.1 ± 3.0 (2.1) 2.1 ± 3.0 (2.1)
Nymphs-3 4.3 ± 2.9 6.0 ± 2.0 2.7 ± 2.7 1.3 ± 1.0 10.0 ± 12.9 (5.7) 1.4 ± 1.0 4.1 ± 3.5
Moulting (%) Nymphs-3 60.5 ± 8.9 61.1 ± 9.8 59.8 ± 9.0 42.3 ± 3.0 (30.1)** 45.5 ± 15.2 (24.8)** 38.1 ± 10.7 (37.1)** 31.8 ± 19.8 (47.4)**
Oviposition (no. eggs/female) Females 66.4 ± 6.0 67.2 ± 8.5 65.6 ± 3.5 61.6 ± 3.4 (7.2) 50.0 ± 4.5 (24.7)** 48.0 ± 6.1 (27.7)** 54.2 ± 6.0 (18.4)**
Fertility (no. nymphs/female) Females 55.5 ± 5.7 56.8 ± 6.3 54.3 ± 5.3 53.7 ± 1.6 (3.2) 32.5 ± 4.4 (41.4)** 36.6 ± 2.7 (34.1)** 41.5 ± 6.7 (25.2)**
Efficacy (%)      30.2 56.0 57.5 57.8
  1. Notes: Results are shown as mean ± standard deviation for each rabbit group. Means were compared between ticks fed on vaccinated and control rabbits (merged) by one-way ANOVA followed by the Dunnett’s t-test. In parentheses, % of change in the corresponding parameter respect to the control: percentage of reduction for ingested blood, moulting, oviposition and fertility; increase for mortality
  2. ** P < 0.01