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Table 3 Effect of the vaccination with recombinant proteins CHI-GST, TSP1-GST + TSP2, RPP0, and PK4 administered in Montanide ISA 50 V2 on O. moubata specimens fed on control and vaccinated rabbits

From: In silico selection of functionally important proteins from the mialome of Ornithodoros erraticus ticks and assessment of their protective efficacy as vaccine targets

Parameter

Developmental stage

Control Merged

Control Montanide

Control SjGST

CHI-GST (% reduction)

TSP1-GST + TSP2 (% reduction)

RPP0 (% reduction)

PK4 (% reduction)

Ingested blood (mg)

Males

32.2 ± 6.0

32.1 ± 5.1

32.3 ± 6.5

29.4 ± 0.4 (8.4)

30.9 ± 1.1 (3.8)

30.1 ± 2.5 (6.2)

26.4 ± 1.0 (17.8)*

Females

203.6 ± 20.4

204.9 ± 20.7

202.3 ± 20.1

159.0 ± 133.0 (22.4)*

198.1 ± 14.6 (3.3)

193.6 ± 18.0 (5.5)

192.2 ± 16.0 (6.2)

Nymphs-3

45.8 ± 6.1

45.8 ± 4.5

45.9 ± 7.0

41.5 ± 2.9 (9.3)

44.1 ± 1.2 (3.6)

45.6 ± 5.7 (0.5)

37.3 ± 1.2 (18.6)*

Mortality (%)

Males

6.4 ± 4.5

5.9 ± 7.1

6.8 ± 0.3

6.7 ± 7.3

6.7 ± 2.8

6.0 ± 8.5

4.4 ± 1.5

Females

5.5 ± 2.7

6.6 ± 0

4.3 ± 3.3

6.3 ± 0

4.2 ± 3.0

2.1 ± 3.0

2.1 ± 3.0

Nymphs-3

2.0 ± 1.7

2.0 ± 2.0

2.0 ± 1.7

0.7 ± 1.0

3.3 ± 3.4 (1.3)

0.7 ± 0.9

1.9 ± 1.6

Moulting (%)

Nymphs-3

67.2 ± 1.7

66.2 ± 1.7

68.2 ± 1.3

63.4 ± 36.5 (4.1)

61.8 ± 8.4 (6.6)

72.0 ± 4.0

57.4 ± 3.6 (13.3)*

Oviposition (no. eggs/female)

Females

197.3 ± 18.8

203.0 ± 23.0

191.0 ± 12.0

158.0 ± 2324 (22.2)**

182.0 ± 14.0 (10.3)

192.0 ± 14.0 (5.4)

191.0 ± 6.7 (5.9)

Fertility (no. nymphs/female)

Females

179.9 ± 15.8

181.0 ± 21.1

179.0 ± 20.0

148.0 ± 21.0 (18.2)*

170.0 ± 15.0 (6.0)

179.0 ± 20.0 (1.1)

180.0 ± 7.0 (0.5)

Efficacy (%)

    

19.6

11.1

0

8.1

  1. Notes: Results are shown as mean ± standard deviation for each rabbit group. Means were compared between ticks fed on vaccinated and control rabbits (treated with adjuvant alone) by one-way ANOVA followed by the Dunnett’s t-test. In parentheses, % of change in the corresponding parameter respect to the control: percentage of reduction for ingested blood, moulting, oviposition and fertility; increase for mortality
  2. * P < 0.05, ** P < 0.01