Marker
|
Location
|
Primary antibody
|
Secondary antibody
|
Labelled cella
|
References
|
---|
Clone
|
Isotype
|
Dilution
|
Isotype (Alexa fluor 488)
|
Dilution
| |
---|
Vimentin
|
Intracellular
|
LN-6d
|
Mouse monoclonal IgM
|
1:100
|
Anti Mouse IgG
|
1:500
|
ECs/Fb
|
[39, 42]
|
Cytokeratin
|
Intracellular
|
C-11d
|
Mouse monoclonal IgG1
|
1:100
|
Anti Mouse IgG
|
1:500
|
ECs/Fb
|
[43,44,45]
|
CD34b**
|
Surface
|
NAe
|
Rabbit polyclonal IgG
|
1:100
|
Anti Rabbit IgG
|
1:500
|
ECs
|
[35]
|
CD44
|
Surface
|
IM7f
|
Rat monoclonal IgG2b
|
1:50
|
Anti Rat IgG
|
1:500
|
ECs/Fb
|
[36, 38]
|
CD31
|
Surface
|
CO.3E-1D4g
|
Mouse monoclonal IgG2a-APCc
|
1:50
|
–
|
–
|
ECs
|
[23, 35]
|
- aECs: Endothelial cells / Fb: Fibroblasts
- bCD34: marker for hematopoietic stem cells [35]
- cCD31 conjugated to Allophycocyanin (APC) (direct staining)
- dSupplied by Sigma-Aldrich
- eSupplied by Biorbyt
- fSupplied by Bio-Rad
- gSupplied by NovusBio