Lytic cycle of Besnoitia besnoiti tachyzoites displays similar features in primary bovine endothelial cells and fibroblasts

Table 1 Markers employed to characterize primary BAEC and bovine fibroblasts by flow cytometry

Marker	Location	Primary antibody			Secondary antibody		Labelled cell^a	References
Marker	Location	Clone	Isotype	Dilution	Isotype (Alexa fluor 488)	Dilution		References
Vimentin	Intracellular	LN-6^d	Mouse monoclonal IgM	1:100	Anti Mouse IgG	1:500	ECs/Fb	[39, 42]
Cytokeratin	Intracellular	C-11^d	Mouse monoclonal IgG1	1:100	Anti Mouse IgG	1:500	ECs/Fb	[43,44,45]
CD34^b**	Surface	NA^e	Rabbit polyclonal IgG	1:100	Anti Rabbit IgG	1:500	ECs	[35]
CD44	Surface	IM7^f	Rat monoclonal IgG2b	1:50	Anti Rat IgG	1:500	ECs/Fb	[36, 38]
CD31	Surface	CO.3E-1D4^g	Mouse monoclonal IgG2a-APC^c	1:50	–	–	ECs	[23, 35]

ISSN: 1756-3305