Skip to main content

Table 1 Tm values of average melting curve peak for each Babesia spp.

From: High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis

Species

Tm value range (mean Tm ± SD) (°C)

DNAa

Variable amount of DNAb

B. bovis

84.15 ± 0.04

84.16 ± 0.19

B. bigemina

80.02 ± 0.02

80.04 ± 0.12

B. major

80.54 ± 0.03

80.62 ± 0.15

B. ovata

81.02 ± 0.05

81.24 ± 0.25

Theileria spp.

79.20 ± 0.19

79.24 ± 0.24

  1. aThe Tm values obtained from HRM using 20 ng of genomic DNA in 3 repeat reactions in 3 independent experiments
  2. bThe Tm values obtained from HRM using a variable amount of genomic DNA ranging from 5 to 50 ng in 3 repeat reactions in 3 independent experiments
Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us