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Table 1 Tm values of average melting curve peak for each Babesia spp.

From: High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis

Species Tm value range (mean Tm ± SD) (°C)
DNAa Variable amount of DNAb
B. bovis 84.15 ± 0.04 84.16 ± 0.19
B. bigemina 80.02 ± 0.02 80.04 ± 0.12
B. major 80.54 ± 0.03 80.62 ± 0.15
B. ovata 81.02 ± 0.05 81.24 ± 0.25
Theileria spp. 79.20 ± 0.19 79.24 ± 0.24
  1. aThe Tm values obtained from HRM using 20 ng of genomic DNA in 3 repeat reactions in 3 independent experiments
  2. bThe Tm values obtained from HRM using a variable amount of genomic DNA ranging from 5 to 50 ng in 3 repeat reactions in 3 independent experiments