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Table 2 Comparison of detection and discrimination results for Babesia spp. in experimentally infected animal samples and field samples

From: High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis

Sample source

Diagnostic method

HRM assay

nPCR assay and 18S rRNA gene sequencing

Experimentally infected samples

B. bovis (n = 25)

B. bovis (n = 25)

B. bigemina (n = 25)

B. bigemina (n = 25)

Field samples

B. bovis (n = 31)

B. bovis (n = 33)

B. bigemina (n = 20)

B. bigemina (n = 20)

B. major (n = 4)

B. major (n = 4)

B. ovata (n = 5)

B. ovata (n = 5)

B. bovis + B. bigemina (n = 3)

B. bovis + B. bigemina (n = 3)

B. bovis + B. ovata (n = 2)

B. bovis + B. ovata (n = 2)

B. bigemina + B. ovata (n = 1)

B. bigemina + B. ovata (n = 1)

B. bovis + B. major (n = 1)

B. bovis + B. major (n = 1)

B. bovis + B. bigemina + B. ovata (n = 1)

B. bovis + B. bigemina + B. ovata (n = 1)

B. bovis, B. bigemina + B. major (n = 0)

B. bovis + B. bigemina + B. major (n = 1)