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Table 2 Comparison of detection and discrimination results for Babesia spp. in experimentally infected animal samples and field samples

From: High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis

Sample source Diagnostic method
HRM assay nPCR assay and 18S rRNA gene sequencing
Experimentally infected samples B. bovis (n = 25) B. bovis (n = 25)
B. bigemina (n = 25) B. bigemina (n = 25)
Field samples B. bovis (n = 31) B. bovis (n = 33)
B. bigemina (n = 20) B. bigemina (n = 20)
B. major (n = 4) B. major (n = 4)
B. ovata (n = 5) B. ovata (n = 5)
B. bovis + B. bigemina (n = 3) B. bovis + B. bigemina (n = 3)
B. bovis + B. ovata (n = 2) B. bovis + B. ovata (n = 2)
B. bigemina + B. ovata (n = 1) B. bigemina + B. ovata (n = 1)
B. bovis + B. major (n = 1) B. bovis + B. major (n = 1)
B. bovis + B. bigemina + B. ovata (n = 1) B. bovis + B. bigemina + B. ovata (n = 1)
B. bovis, B. bigemina + B. major (n = 0) B. bovis + B. bigemina + B. major (n = 1)