Table 4 Partial of upregulated DEGs in the high throughput sequencing data

Iigp1

Interferon-inducible GTPase 1

6.5608

Required for disruption of the parasitophorous vacuole formed following T. gondii infection and subsequent killing of the parasite

Klrb1c

Killer cell lectin-like receptor subfamily B member 1C

6.17868

Plays a stimulatory role on natural killer (NK) cells cytotoxicity, activation by cross-linking of the receptor induces Ca²⁺ mobilization and interferon-gamma production

Tgtp1

T cell-specific GTPase 1

5.61362

Involved in innate cell-autonomous resistance to intracellular pathogens, such as T. gondii

Tnfsf10

Tumor necrosis factor (ligand) superfamily, member 10

1.53097

Involved in male gonad development and regulation of the apoptotic process

Spata18

Spermatogenesis associated 18

1.29567

A key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage

Pgk2

Phosphoglycerate kinase 2

1.11099

Essential for sperm motility and male fertility but is not required for the completion of spermatogenesis

Piwil2

Piwi-like RNA-mediated gene silencing 2

1.1907

Essential role in meiotic differentiation of spermatocytes and self-renewal of spermatogonial stem cells