Fig. 11

Colonization of cardiac tissue with T. cruzi amastigotes. Male Wistar rats were infected with T. cruzi and treated for 14 days with ghrelin. On day 15 post-infection, the animals were euthanized, and the hearts collected. The cardiac tissue was fixed with 10% buffered formaldehyde, paraffin-embedded and prepared for hematoxylin-eosin staining. Arrows indicate the amastigote nests. a Cardiac tissue from infected and non-treated animals (400×). b Cardiac tissue from infected and ghrelin-treated animals (400×). c Cardiac tissue from infected and non-treated animals (1000×). d Cardiac tissue from infected and ghrelin-treated animals (1000×). Scale-bars: a, b 100 µm; c, d, 50 µm