Fig. 3From: Effects of ghrelin supplementation on the acute phase of Chagas disease in ratsNitric oxide quantification. Male Wistar rats were infected with T. cruzi and treated for 14 days with ghrelin. On day 15 post-infection, the animals were euthanized and the peritoneal exudate collected. Peritoneal cells (1 × 106 cells/ml) were incubated with LPS for 48 h and the nitric oxide measured by Griess method, using serial dilutions of sodium nitrite as a reference. Abbreviations: C, non-infected and non-treated control; CG, non-infected and treated control; I, infected and non-treated animals; IG, infected and treated animals. *P < 0.05Back to article page