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Fig. 3 | Parasites & Vectors

Fig. 3

From: Effects of ghrelin supplementation on the acute phase of Chagas disease in rats

Fig. 3

Nitric oxide quantification. Male Wistar rats were infected with T. cruzi and treated for 14 days with ghrelin. On day 15 post-infection, the animals were euthanized and the peritoneal exudate collected. Peritoneal cells (1 × 106 cells/ml) were incubated with LPS for 48 h and the nitric oxide measured by Griess method, using serial dilutions of sodium nitrite as a reference. Abbreviations: C, non-infected and non-treated control; CG, non-infected and treated control; I, infected and non-treated animals; IG, infected and treated animals. *P < 0.05

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