Skip to main content

Advertisement

Fig. 6 | Parasites & Vectors

Fig. 6

From: Effects of ghrelin supplementation on the acute phase of Chagas disease in rats

Fig. 6

Evaluation of T cell proliferation by CFSE. Male Wistar rats were infected with T. cruzi and treated for 14 days with ghrelin. On day 15 post-infection, the animals were euthanized and the spleens collected. The splenic cells were processed, labelled with CFSE and incubated with ConA, T. cruzi extract or RPMI (control) for 4 days, 37 °C, 5% CO2. In parallel, the same approach was performed with incubation for 15 min (day 0). The T cell population was distinguished using anti-rat CD3+/APC antibody and the proliferation analyzed by flow cytometry. a Proliferation for 15 min (day 0) with RPMI. b Proliferation for 4 days (day 4) with RPMI. c Proliferation for 15 min (day 0) with ConA. d Proliferation for 4 days (day 4) with ConA. e Proliferation for 15 min (day 0) with T. cruzi extract. f Proliferation for 4 days (day 4) with T. cruzi extract. Abbreviations: C, non-infected and non-treated control; CG, non-infected and treated control; I, infected and non-treated animals; IG, infected and treated animals. *P < 0.05

Back to article page