Fig. 7From: Effects of ghrelin supplementation on the acute phase of Chagas disease in ratsEarly and late apoptosis of splenic cells. Male Wistar rats were infected with T. cruzi and treated for 14 days with ghrelin. On day 15 post-infection, the animals were euthanized and the spleens collected. The splenic cells were processed, labelled with CFSE and incubated with T. cruzi extract or RPMI (control) for 24 h, 37 °C, 5% CO2. In parallel, the same approach was performed with incubation for 15 min (day 0). The cells were incubated with Annexin-V FITC for 15 min, at room temperature in the dark. Before the analysis by flow cytometry, propidium iodide was added. a Early apoptosis (AnnexinV+PI-) of cells incubated for 15 min with RPMI. b Late apoptosis (AnnexinV+PI+) of cells incubated for 15 min with RPMI. c Early apoptosis (AnnexinV+PI−) of cells incubated for 24 h with RPMI. d Early apoptosis (AnnexinV+PI−) of cells incubated for 24 h with T. cruzi extract. e Late apoptosis (AnnexinV+PI+) of cells incubated for 24 h with RPMI. f Late apoptosis (AnnexinV+PI+) of cells incubated for 24 h with T. cruzi extract. Abbreviations: C, non-infected and non-treated control; CG, non-infected and treated control; I, infected and non-treated animals; IG, infected and treated animals. *P < 0.05Back to article page