Fig. 1

Expression of Tim-3 on splenic CD3⁺CD8⁺, CD3^-CD49b⁺, CD3⁺CD4⁺ and CD3⁻CD19⁺ cells, and the proportional changes in P. berghei-infected mice. Tim-3 expression on splenic CD3⁺CD8⁺ T cells (a, e), CD3^-CD49b⁺ cells (b, f), CD3⁺CD4⁺ cells (c, g), CD3⁻CD19⁺ cells (d, h) and the proportions of these cells (i-p) were detected by flow cytometry at days 0, 4, 9, 11, 13, and 14 p.i. (a–d) Representative FACS plots of Tim-3 expression on CD3⁺CD8⁺ T cells, CD3⁻CD49b⁺ cells, CD3⁺CD4⁺ T cells and CD3⁻CD19⁺ cells. (e–h) Histograms showing the proportion of Tim-3⁺ cells within the CD3⁺CD8⁺ T cells, CD3⁻CD49b⁺ cells, CD3⁺CD4⁺ T cells and CD3⁻CD19⁺ cells. (i–l) Representative FACS plots of CD3⁻CD49b⁺ cells, CD3⁺CD8⁺ T cells, CD3⁺CD4⁺ T cells and CD3⁻CD19⁺ cells in splenic lymphocytes. (m–p) Histograms showing the proportion of CD3⁻CD49b⁺ cells, CD3⁺CD8⁺ T cells, CD3⁺CD4⁺ T cells and CD3⁻CD19⁺ cells in splenic lymphocytes. The results are representative of three independent experiments with five to seven mice in each group per experiment, with data denoting means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001, *indicates comparisons with the day 0 group