First records of tick-borne pathogens in populations of the taiga tick Ixodes persulcatus in Sweden

Jaenson, Thomas G. T.; Wilhelmsson, Peter

doi:10.1186/s13071-019-3813-0

Parasites & Vectors

Table 1 Primers and probes used for molecular analysis of tick-borne pathogens and Ixodes ticks

From: First records of tick-borne pathogens in populations of the taiga tick Ixodes persulcatus in Sweden

Organism	Target	Primer/Probe name	Sequence (5′–3′)	Amplicon length (bp)	References
A. phagocytophilum	gltA	ApF	TTTTGGGCGCTGAATACGAT	64	Henningsson et al. [33]
		ApR	TCTCGAGGGAATGATCTAATAACGT
		ApM	FAM-TGCCTGAACAAGTTATG-BHQ1
Babesia spp.	18S rRNA	BJ1	GTCTTGTAATTGGAATGATGG	411–452	Casati et al. [39]
Babesia spp.	18S rRNA	BN2	TAGTTTATGGTTAGGACTACG	411–452	Casati et al. [39]
Borrelia spp.	16S rRNA	B16S_FL	GAGTCGTCAAGACTGACGCTAAGTC	131	Wilhelmsson et al. [26]
	16S rRNA	B16S_R	GCACACTTAACACGTTAGCTTCGGTACTAAC	131	Wilhelmsson et al. [26]
	5S–23S rRNA IGS	B5S-23S_F	CTGCGAGTTCGCGGGAGA	225–266	Postic et al. [27]
		B5S-23S_R	TCCTAGGCATTCACCATA		Postic et al. [27]
		B5S-23S_Fn	GAGTTCGCGGGAGAGTAA		Wilhelmsson et al. [26]
		B5S-23S_Rn	TAGGCATTCACCATAGACTCTT		Wilhelmsson et al. [26]
Borrelia miyamotoi	flaB	Bm_F	AGAAGGTGCTCAAGCAG	156	Hovius et al. [28]
		Bm_R	TCGATCTTTGAAAGTGACATAT
		Bm_P	FAM-AGCACAACAGGAGGGAGTTCAAGC-BHQ1
N. mikurensis	16S rRNA	Neo_16S_F	GTAAAGGGCATGTAGGCGGTTTAA	107	Labbé Sandelin et al. [32]
N. mikurensis	16S rRNA	Neo_16S_R	TCCACTATCCTCTCTCGATCTCTAGTTTAA	107	Labbé Sandelin et al. [32]
Rickettsia spp.	gltA	CS-F	TCGCAAATGTTCACGGTACTTT	74	Stenos et al. [34]
		CS-R	TCGTGCATTTCTTTCCATTGTG
		CS-P	FAM-TGCAATAGCAAGAACCGTAGGCTGGATG-BHQ1
	17kDA	Rr17kDa.61p	GCTCTTGCAACTTCTATGTT	434	Carl et al. [35]
	17kDA	Rr17kDa.492n	CATTGTTCGTCAGGTTGGCG	434	Carl et al. [35]
	ompB	Rc.rompB.4362p	GTCAGCGTTACTTCTTCGATGC	475	Choi et al. [36]
		Rc.rompB.4,836n	CCGTACTCCATCTTAGCATCAG	475
		Rc.rompB.4,496p	CCAATGGCAGGACTTAGCTACT	267
		Rc.rompB.4,762n	AGGCTGGCTGATACACGGAGTAA	267
	gltA	RH314	AAACAGGTTGCTCATCATTC	837	Wallménius et al. [37]
		CSF-R	AAGTACCGTGAACATTTGCGA	837
		CS-Ric-R	CAGTGAACATTTGCGACGGTA	832
		CS535d	GCAATGTCTTATAAATATTC	Sequencing primer
TBEV	11,054–11,121^a	F-TBE 1	GGGCGGTTCTTGTTCTCC	68	Schwaiger & Cassinotti [30]
		R-TBE 1	ACACATCACCTCCTTGTCAGACT
		TBE-probe-WT	FAM-TGAGCCACCATCACCCAGACACA-BHQ1
	1329–1416^a	TBEE-F6	GGCTTGTGAGGCAAAAAAGAA	88	Gäumann et al. [31]
		TBEE-R2	TCCCGTGTGTGGTTCGACTT
		TBEE-P4	HEX-AAGCCACAGGACATGTGTACGACGCC-BHQ1
Ixodes sp.	ITS2	IXO-I2-F4	TCTCGTGGCGTTGATTTGC	95	Sormunen et al. [25]
		IXO-I2-R4	CTGACGGAAGGCTACGACG
		Ipe-I2-P4	FAM-TGCGTGGAAAGAAAACGAG-BHQ1
		Iri-I2-P4	VIC-TGCTCGAAGGAGAGAACGA-BHQ1

^aGenome region of TBEV strain Neudoerfl (U27495)
Abbreviations: FAM, 6-carboxy-fluorescine; HEX, 6-carboxy-hexachlorofluoriscine; VIC, 6-carboxy-rhodamine; BHQ, Black Hole Quencher; IGS, intergenic spacer; ITS, internal transcribed spacer

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ISSN: 1756-3305

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