Table 1 Primer and probe sequences and concentrations used in duplex TaqMan qPCR assay for detection of T. cruzi DNA in mammalian reservoir species
Gene target | Primer/probe name | Primer/probe sequence (5’-3’) | Reaction concentration (µM) | Amplicon size (bp) | Source |
|---|---|---|---|---|---|
Satellite repeat unit from T. cruzi genome | Primer Cruzi 1c | TGAATGGYGGGAGTCAGAG | 0.75 | 98 | Ramírez et al. [14] |
Primer Cruzi 2c | ATTCCTCCAAGMAGCGGAT | 0.75 | |||
Probe Cruzi 3 | FAM-CACACACTGGACACCAA-NFQ-MGB | 0.05 | |||
IRBP gene from mammalian genome | Primer IRBP2 Fw | CCAAYACVACCACTGAGATCTG | 0.60 | 140 | Present study |
Primer IRBP3 Rv | GCGCATCTGYTTGAGGATGTARG | 0.60 | |||
Probe IRBP Tq | HEX-TGGTGGTCCTCACCAG-NFQ-MGB | 0.05 |