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Table 1 Primer and probe sequences and concentrations used in duplex TaqMan qPCR assay for detection of T. cruzi DNA in mammalian reservoir species

From: Development and evaluation of a duplex TaqMan qPCR assay for detection and quantification of Trypanosoma cruzi infection in domestic and sylvatic reservoir hosts

Gene targetPrimer/probe namePrimer/probe sequence (5’-3’)Reaction concentration (µM)Amplicon size (bp)Source
Satellite repeat unit from T. cruzi genomePrimer Cruzi 1cTGAATGGYGGGAGTCAGAG0.7598Ramírez et al. [14]
Primer Cruzi 2cATTCCTCCAAGMAGCGGAT0.75 
Probe Cruzi 3FAM-CACACACTGGACACCAA-NFQ-MGB0.05 
IRBP gene from mammalian genomePrimer IRBP2 FwCCAAYACVACCACTGAGATCTG0.60140Present study
Primer IRBP3 RvGCGCATCTGYTTGAGGATGTARG0.60 
Probe IRBP TqHEX-TGGTGGTCCTCACCAG-NFQ-MGB0.05