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Table 5 Parasite loads in satDNA/IRBP qPCR-positive samples from mammalian reservoir hosts

From: Development and evaluation of a duplex TaqMan qPCR assay for detection and quantification of Trypanosoma cruzi infection in domestic and sylvatic reservoir hosts

Sample IDMammalian speciesDuplex SatDNA/IRBP qPCRDTU
Mean Cq IRBPMean Cq cruzipar.eq/mllog par.eq/ml
1Felis catus25.5928.700.30− 0.522878745TcI
2Felis catus27.1328.520.36− 0.443697499TcI
3Felis catus27.8224.789.490.977266212TcI
4Felis catus26.5324.739.940.997386384TcI
5Felis catus26.3923.7523.721.375114685TcI
6Felis catus26.1123.9619.671.29380436TcI
7Felis catus25.9625.5866.401.822168079TcI
8Felis catus26.7020.89296.452.471951455TcI
9Felis catus25.8421.58160.172.204581176TcI
10Felis catus26.4821.40187.502.273001272TcI
11Ovis ariesa31.6129.450.14− 0.853871964TcI/TcVI
12Ovis ariesa33.7429.130.21− 0.677780705TcI/TcVI
13Canis familiarisa29.6027.470.84− 0.075720714TcVI
14Canis familiarisa28.8329.310.18− 0.744727495TcI/TcVI
15Canis familiarisa24.6929.270.19− 0.721246399TcI/TcVI
16Canis familiaris35.9218.23402.732.60500859TcI
17Didelphis marsupialis26.0126.800.96− 0.017728767nd
18Didelphis marsupialis26.5421.2946.411.666564777nd
19Didelphis marsupialis26.3022.0427.391.437592032nd
20Marmosa murina32.7629.280.17− 0.782516056nd
21Marmosa murina32.3729.170.21− 0.688246139nd
22Marmosa murina24.0718.72284.382.453891414nd
  1. a DNA extracted with phenol-chloroform
  2. Abbreviation: nd, not determined