Establishment of an innovative and sustainable PCR technique for 1534 locus mutation of the knockdown resistance (kdr) gene in the dengue vector Aedes albopictus

Table 5 The genotypes and frequencies of Ae. albopictus kdr gene at the 1534 locus

Methods	Sample size	Genotype			Genotype frequency			Allele frequency
Methods	Sample size	SS	RS	RR	SS%	RS%	RR%	S%	R%
Sequencing	165	30	82	53	18.18	49.70	32.12	43.03	56.97
AS-PCR	165	30	81	54	18.18	49.09	32.73	42.73	57.27

Abbreviations: SS, susceptible homozygote; SS%, SS/(SS + RS + RR) × 100%; RS, wild/mutant heterozygote; RS%, RS/(SS + RS + RR) × 100%; RR, mutant heterozygote and mutant homozygote; RR%, RR/(SS + RS + RR) × 100%; S%, SS% + 0.5 × RS%; R%, 1 − S%

ISSN: 1756-3305