Table 1 Analysis results for the variance immature life history traits and physiological performance of Ae. aegypti males reared with various combinations of larval diets and temperature regimes

L1

Temp

14.034

4

3.508

1982.741

< 0.0001

Diet

0.827

1

0.827

467.538

< 0.0001

Temp * Diet

0.205

4

0.051

29.002

< 0.0001

L2

Temp

32.972

4

8.243

709.500

< 0.0001

Diet

0.000

1

0.000

0.028

0.867

Temp * Diet

1.199

4

0.300

25.792

< 0.0001

L3

Temp

30.814

4

7.704

703.912

< 0.0001

Diet

0.407

1

0.407

37.172

< 0.0001

Temp * Diet

0.147

4

0.037

3.354

0.010

L4

Temp

29.466

4

7.367

2036.100

< 0.0001

Diet

0.253

1

0.253

69.847

< 0.0001

Temp * Diet

0.588

4

0.147

40.629

< 0.0001

Larval stage

Temp

23.455

4

5.864

5623.814

< 0.0001

Diet

0.365

1

0.365

350.346

< 0.0001

Temp * Diet

0.192

4

0.048

45.942

< 0.0001

Pupal stage

Temp

16.643

4

4.161

1262.635

< 0.0001

Diet

0.001

1

0.001

0.337

0.562

Temp * Diet

0.144

4

0.036

10.894

< 0.0001

Total immature

Temp

14.115

4

3.529

5600.410

< 0.0001

Diet

0.140

1

0.140

222.739

< 0.0001

Temp * Diet

0.098

4

0.025

39.062

< 0.0001

Pupation (%)

Temp

0.759

4

0.190

5.692

0.002

Diet

0.189

1

0.189

5.685

0.024

Temp * Diet

0.519

4

0.130

3.889

0.012

Adult eclosion (%)

Temp

4.291

4

1.073

22.362

< 0.0001

Diet

0.140

1

0.140

2.921

0.098

Temp * Diet

0.671

4

0.168

3.499

0.018

Cephalothorax length

Temp

1.160

4

0.290

92.882

< 0.0001

Diet

0.026

1

0.026

8.424

0.004

Temp * Diet

0.043

4

0.011

3.445

0.010

Wing length

Temp

10.374

4

2.594

385.580

< 0.0001

Diet

0.060

1

0.060

8.952

0.003

Temp * Diet

0.132

4

0.033

4.911

0.001

Adult longevity (water-fed)

Temp

0.327

4

0.082

14.617

< 0.0001

Diet

4.973

1

4.973

888.087

< 0.0001

Temp * Diet

0.828

4

0.207

36.978

< 0.0001

Adult longevity (sugar-fed)

Temp

1.383

4

0.346

10.219

< 0.0001

Diet

0.933

1

0.933

27.576

< 0.0001

Temp * Diet

0.152

4

0.038

1.125

0.346

Glucose

Temp

1.416

4

0.354

16.999

< 0.0001

Diet

0.217

1

0.217

10.421

0.002

Temp * Diet

0.141

4

0.035

1.691

0.170

Glycogen

Temp

3.539

4

0.885

36.564

< 0.0001

Diet

0.165

1

0.165

6.814

0.013

Temp * Diet

0.814

4

0.204

8.410

< 0.0001

Trehalose

Temp

0.154

4

0.038

0.976

0.431

Diet

0.732

1

0.732

18.606

< 0.0001

Temp * Diet

0.160

4

0.040

1.015

0.411

Female insemination

Temp

0.010

4

0.003

0.270

0.895

Diet

0.016

1

0.016

1.671

0.206

Temp * Diet

0.021

4

0.005

0.544

0.705