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Table 4 Energy metabolic reserves and temperature tolerance of Ae. aegypti males reared with various combinations of larval diets and temperature regimes

From: Effects of larval diets and temperature regimes on life history traits, energy reserves and temperature tolerance of male Aedes aegypti (Diptera: Culicidae): optimizing rearing techniques for the sterile insect programmes

Larval diet

T (°C)

Sugar content (µg/mg insect weight)

Temperature tolerance (°C)

Glucose

Glycogen

Trehalose

ULT50

LLT50

Khan’s

15

7.14 ± 0.65a

3.46 ± 0.31a

7.53 ± 0.63a

30.42 (–)

n.a.

20

7.90 ± 1.50a

3.73 ± 0.68a

8.75 ± 1.44a

36.44 (28.72–38.65)

− 1.43 (− 1.66– − 1.26)

25

8.87 ± 0.74a

2.74 ± 0.19a

8.27 ± 3.06a

41.74 (32.99–44.08)

− 1.11 (− 2.13– − 1.00)

28

12.78 ± 2.36a

8.68 ± 1.18b

9.17 ± 3.42a

42.06 (40.80–43.09)

− 1.80 (− 3.23–0.29)

32

27.96 ± 3.82b

29.97 ± 7.50c

12.38 ± 1.82a

42.79 (42.42–43.17)

− 1.21 (− 1.52– − 0.87)

IAEA 2

15

5.21 ± 0.40a

2.19 ± 0.24a

11.33 ± 1.38a

na

na

20

6.48 ± 0.38ab

3.11 ± 0.59ab

14.11 ± 0.68a

40.01 (39.701-40.321)

− 2.10 (-2.25– − 1.96)

25

9.74 ± 3.61ab

6.88 ± 1.32c

18.95 ± 2.99a

41.41 (39.62–42.55)

− 2.32 (− 2.46– − 2.19)

28

9.56 ± 2.70ab

5.60 ± 0.47bc

15.00 ± 2.08a

42.2 (41.28–43.06)

− 1.05 (–)

32

12.82 ± 2.01b

8.54 ± 0.91c

16.39 ± 4.58a

42.93 (41.23–45.82)

− 0.29 (–)

  1. Abbreviations: na, not applicable due to mortality of > 90% for each test temperature; (–), fiducial limit not generated due to high mortality occurring when temperature decreased; T, temperature
  2. Notes: Mean values followed by the same letter in the same column within each larval diet are not significantly different, Tukey’s (HSD) (P < 0.05). Temperature tolerance was analyzed using probit analysis