Morphology is not a reliable taxonomic tool for the genus Lernaea: molecular data and experimental infection reveal that L. cyprinacea and L. cruciata are conspecific

Table 4 Pairwise identity (below the diagonal) and variable sites (above the diagonal) for the 18S dataset

Sequence		1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17
1	Lcy1_w		1	0	0	0	0	0	0	0	1	1	2	2	2	2	1	0
2	Lcy2_w	99.9		1	1	1	1	1	1	1	2	2	3	3	2	3	2	1
3	Lcy3_w	100	99.9		0	0	0	0	0	0	1	1	2	2	2	2	1	0
4	Lcy4_w	100	99.9	100		0	0	0	0	0	1	1	2	2	2	2	1	0
5	Lcy5_w	100	99.9	100	100		0	0	0	0	1	1	2	2	2	2	1	0
6	Lcy6_l	100	99.9	100	100	100		0	0	0	1	1	2	2	2	2	1	0
7	Lcy7_l	100	99.9	100	100	100	100		0	0	1	1	2	2	2	2	1	0
8	Lcr1_l	100	99.9	100	100	100	100	100		0	1	1	2	2	2	2	1	0
9	Lcr2_l	100	99.9	100	100	100	100	100	100		1	1	2	2	2	2	1	0
10	Lcr4_w	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9		2	3	3	3	3	2	1
11	Lcr5_w	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9		3	3	3	3	2	1
12	Lcr6_w	99.9	99.8	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.8	99.8		4	4	4	3	2
13	LcyGB	99.9	99.8	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.8	99.8	99.7		2	2	3	2
14	LcyGB	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.8	99.8	99.7	99.9		2	3	2
15	LcyGB	99.9	99.8	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.8	99.8	99.7	99.9	99.9		3	2
16	LcyGB	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.9	99.8	99.8	99.8	99.8		1
17	LcyGB	100	99.9	100	100	100	100	100	100	100	99.9	99.9	99.9	99.9	99.9	99.9	99.9

Notes: Lower left half shows pairwise identity (the % of bases/residues that are identical), and upper right half shows the number of variable sites. Sequences 1–12 belong to specimens from this study, and numbers 13–17 (LcyGB) are from GenBank: DQ107554-DQ107557 and KP235363, respectively
Abbreviations: Lcy, Lernaea cyprinacea; Lcr, L. cruciate; w, specimen collected in the wild; l, specimen collected from the laboratory infection

ISSN: 1756-3305