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Fig. 3 | Parasites & Vectors

Fig. 3

From: A new methodology for sporogony research of avian haemoproteids in laboratory-reared Culicoides spp., with a description of the complete sporogonic development of Haemoproteus pastoris

Fig. 3

Main procedures of exposure of Culicoides nubeculosus colony to Haemoproteus pastoris (lineage hLAMPUR01) infection by allowing the insects to take blood meal on naturally infected common starling Sturnus vulgaris (a, b). Note that exposure was done by gentle touching of a cardboard box (arrow) with biting midges to the pectoral muscle of the infected bird (a) and then engorged insects are released into a larger insect cage (b) (note that a zipper is sewed into one side of the cage) long arrow, insect cage; triangle arrowhead, zipper. Exposed females (c) were maintained in insect cage made of bolting silk (b) and their midguts were dissected (d) (arrow, dissected midgut full with blood). Mature gametocytes (e, f) were present in the peripheral blood of the infected starling (arrowheads, parasite nuclei), and the sporogonic stages (g–i) developed in exposed female biting midges: macrogametocyte (e); microgametocyte (f); ookinete (g) (arrowhead, parasite nuclei); oocyst (h) (arrowhead, oocyst); sporozoite (i) (arrowhead, parasite nuclei). Giemsa (e–g, i) and mercurochrome (h) stained preparations. Scale-bars: 10 µm

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Parasites & Vectors

ISSN: 1756-3305

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