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Fig. 4 | Parasites & Vectors

Fig. 4

From: Vaccination of mice with a recombinant novel cathepsin B inhibits Trichinella spiralis development, reduces the fecundity and worm burden

Fig. 4

Identification of rTsCB. a SDS-PAGE analysis of rTsCB. Lane M: protein marker; Lane 1: lysates of recombinant E. coli carrying pQE-80L/TsCB prior to induction; Lane 2: lysates of recombinant E. coli carrying pQE-80L/TsCB following induction; Lane 3: purified rTSCB (arrow). b Western blot of rTsCB. Muscle larva somatic soluble protein (Lane 1) and ES protein (Lane 2) were recognized by mouse infection sera, but the rTsCB (Lane 3) was not recognized by infection sera. Native TsCB in muscle larva somatic protein (Lane 4) and rTsCB (Lane 6), not present in ES proteins (Lane 5), was detected by anti-TsCB serum. Muscle larva somatic protein (Lane 7), ES protein (Lane 8) and rTsCB (Lane 9) were not detected by normal mouse serum. c. Western blot showing that natural TsCB was detected using anti-TsCB serum in soluble somatic protein of the four life-cycle stages of T. spiralis (Lane 1: muscle larvae; Lane 2: IIL1; Lane 3: 3-day-old adults; Lane 4: NBL)

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