Fig. 5

Location of TsCB of different life-cycle stages of T. spiralis by IIFA using anti-rTsCB antibody. Green fluorescence staining was detected in the cuticle and stichosome of the muscle larvae (a) and IIL1 larvae (b), 3-day-old females and embryos (c). Muscle larva incubated by infection sera was utilized as a positive control (d). Muscle larva incubated with pre-immune sera (e) and PBS (f) were used as negative controls. Scale-bars: 50 μm