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Table 1 Combined results of all spiking experiments with romaine lettuce and strawberries

From: A novel protocol to isolate, detect and differentiate taeniid eggs in leafy greens and berries using real-time PCR with melting curve analysis

Number of eggs spiked

Romaine lettuce

Strawberries

Positive/Total

Mean Cqa

Positive/Total

Mean Cqb

500

30/30

26.49

nd

na

100

16/16

29.09

14/18c

28.09

50

15/15

29.95

1/1

30.09

10

12/12

34.05

9/9

32.36

5

21/21

34.2

10/10

32.8

0

0/9

na

0/8

na

  1. aGlycine and 0.1% Alconox only
  2. b0.1% Alconox only
  3. c3 of 6 samples washed with glycine and 1 of 6 samples washed with sodium pyrophosphate were falsely negative
  4. Notes: All lower spikes for strawberries were washed with 0.1% Alconox
  5. Abbreviations: Cq, quantification cycle; nd, not done; na, not applicable