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Fig. 3 | Parasites & Vectors

Fig. 3

From: Diagnosing bovine parafilariosis: utility of the cytochrome c oxidase subunit 1 gene and internal transcribed spacer region for PCR detection of Parafilaria bovicola in skin biopsies and serohemorrhagic exudates of cattle

Fig. 3

Initial screening amplification of liquid exudate samples using cox1-PCR. Lane 1: marker (MassRuler™ Express Forward DNA Ladder Mix, Thermo Fisher Scientific); Lane 2: no-template control; Lane 3: positive control; Lane 4: 100 µl native liquid exudate (cow 1); Lane 5: 200 µl native liquid exudate (cow 1); Lane 6: 100 µl liquid exudate fixed in 70% ethanol (cow 2); Lane 7: 200 µl liquid exudate fixed in 70% ethanol (cow 2); Lane 8: 100 µl native liquid exudate (cow 2); Lane 9: 200 µl native liquid exudate (cow 2)

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