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Fig. 6 | Parasites & Vectors

Fig. 6

From: Diagnosing bovine parafilariosis: utility of the cytochrome c oxidase subunit 1 gene and internal transcribed spacer region for PCR detection of Parafilaria bovicola in skin biopsies and serohemorrhagic exudates of cattle

Fig. 6

Detection of P. bovicola in samples from presumably affected (Lanes 4–12) and control animals (Lanes 13–14) and a Musca sp. fly using ITS-PCR. Lane 1: marker (MassRuler® Express Forward DNA Ladder Mix, Thermo Fisher Scientific); Lane 2: no-template control; Lane 3: positive control; Lane 4: native liquid exudate (cow 1); Lane 5: native liquid exudate (cow 3); Lane 6: native liquid exudate (cow 4); Lane 7: liquid exudate fixed in 70% ethanol (cow 2); Lane 8: native liquid exudate (cow 2); Lane 9: native liquid exudate (cow 5); Lane 10: dry exudate (cow 2); Lane 11: dry exudate (cow 6); Lane 12: skin biopsy fixed in 70% ethanol (cow 7); Lane 13: EDTA blood (control cow 1); Lane 14: skin biopsy (control cow 2); Lane 15: Musca sp. fly

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Parasites & Vectors

ISSN: 1756-3305

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