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Fig. 3 | Parasites & Vectors

Fig. 3

From: Expression of a rK39 homologue from an Iranian Leishmania infantum isolate in Leishmania tarentolae for serodiagnosis of visceral leishmaniasis

Fig. 3

SDS-PAGE (a) and Western blot (b) showing the protein composition of the different fractions collected from L. tarentolae clone pLEXSY-hyg2-MCAN14/47, before and after the purification on the Ni-NTA. a Lane 1: whole cell lysate; Lane 2: flow-through from Ni-NTA column; Lanes 3 and 4: wash fractions; Lanes 5–8: elution fractions; Lane 9: recombinant His-tagged H2B (positive control); Lane 10: 11–245 kDa protein marker. b Lanes 1–6: elution fractions; Lane 7: flow-through; Lane 8: whole cell lysate; Lane 9: 11–245 kDa protein marker

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Parasites & Vectors

ISSN: 1756-3305

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