Skip to main content

Table 1 Primers used in the PCR

From: Expression of a rK39 homologue from an Iranian Leishmania infantum isolate in Leishmania tarentolae for serodiagnosis of visceral leishmaniasis

Primer

Configuration

Sequence (5′–3′)

XbaI-rK39 F

Secreted

CTGGCGCCTCTCTAGAGCTCGCAACC

NcoI-rK39 F

Cytoplasmic

ACCAGATCTGCCATGGTCGCAACCGAGTGGGAGGA

KpnI-rK39 R

Hexa-histag

TGGTGATGGTGGTGGGTACCACTCGCCAGCTCC

  1. Notes: Nucleotides in italics, sequence complementary to pLEXSY-hyg2; nucleotides in bold, restriction site; nucleotides underlined, K39 sequence from GenBank (L07879)
Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us