Table 1 Primer sequence information for pvetramps for cloning into the pEU expression vector
Gene ID | ORF (bp) | Expression region sequence (bp) | Genic-specific sense primer (5′–3′) | Genic-specific anti-sense primer (5′–3′) |
|---|---|---|---|---|
PVX_090230 | 438 | 238–438 (201) | GGGCGGATATCTCGAGTACTACAAGCAGAAGAAGAGCAAGG | GCGGTACCCGGGATCCTCATTTGGATG GGGGGGT |
PVX_096070 | 549 | 79–627 (549) | GGGCGGATATCTCGAGCATGTAAATAACAAGCCCCACG | GCGGTACCCGGGATCCTTAAGATTCTTGTGGGGTTTCTG |
PVX_086915 | 564 | 76–564 (489) | GGGCGGATATCTCGAGAATTTATTTCTGGAAAAAGTGAAAGC | GCGGTACCCGGGATCCTTATTTACTGTTCGGAATTAAGCTTG |
PVX_088870 | 519 | 76–519 (444) | GGGCGGATATCTCGAGGAGAATGTGGTAAAGAAGAAAGTCCT | GCGGTACCCGGGATCCTCACGTTTGAGTGTCACCAG |
PVX_001715 | 504 | 64–501 (438) | GTTCCGCGTGGATCCATGGAGGAAGTGAAGGCCGTCT | ATGCGGCCGCTCGAGTATAATGGGAGATCCCATAACA |
PVX_003565 | 333 | 67–333 (267) | GGGCGGATATCTCGAGTTCTACAATAATGTTGTAGCAGGAAAG | GCGGTACCCGGGATCCTTATTGGATGTTGCTGCCTTT |